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\n \n\n \n \n Kamata, M., Taniguchi, Y., Yaguchi, J., Tanaka, H., & Yaguchi, S.\n\n\n \n \n \n \n \n Nonmuscular Troponin‐I is required for gastrulation in sea urchin embryos.\n \n \n \n \n\n\n \n\n\n\n Developmental Dynamics,dvdy.680. December 2023.\n \n\n\n\n
\n\n\n\n \n \n \"NonmuscularPaper\n  \n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n  \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n\n\n\n
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@article{kamata_nonmuscular_2023,\n\ttitle = {Nonmuscular {Troponin}‐{I} is required for gastrulation in sea urchin embryos},\n\tissn = {1058-8388, 1097-0177},\n\turl = {https://anatomypubs.onlinelibrary.wiley.com/doi/10.1002/dvdy.680},\n\tdoi = {10.1002/dvdy.680},\n\tabstract = {Abstract\n            \n              Background\n              \n                Gastrulation is one of the most important events in our lives (Barresi and Gilbert, 2020,\n                Developmental Biology\n                , 12th ed.). The molecular mechanisms of gastrulation in multicellular organisms are not yet fully understood, since many molecular, physical, and chemical factors are involved in the event.\n              \n            \n            \n              Results\n              \n                Here, we found that one of muscle components, Troponin‐I (TnI), is expressed in future gut cells, which are not muscular cells at all, and regulates gastrulation in embryos of a sea urchin,\n                Hemicentrotus pulcherrimus\n                . When we block the function of TnI, the invagination was inhibited in spite that the gut‐cell specifier gene is normally expressed. In addition, blocking myosin activity also induced incomplete gastrulation.\n              \n            \n            \n              Conclusion\n              \n                These results strongly suggested that TnI regulates nonmuscular actin–myosin interactions during sea urchin gastrulation. So far, Troponin system is treated as specific only for muscle components, especially for striated muscle, but our data clearly show that TnI is involved in nonmuscular event. It is also reported that recent sensitive gene expression analysis revealed that Troponin genes are expressed in nonmuscular tissues in mammals (Ono et al.,\n                Sci Data\n                , 2017;4:170105). These evidences propose the new evolutionary and functional scenario of the involvement of Troponin system in nonmuscular cell behaviors using actin‐myosin system in bilaterians including human being.},\n\tlanguage = {en},\n\turldate = {2024-04-04},\n\tjournal = {Developmental Dynamics},\n\tauthor = {Kamata, Mai and Taniguchi, Yuri and Yaguchi, Junko and Tanaka, Hiroyuki and Yaguchi, Shunsuke},\n\tmonth = dec,\n\tyear = {2023},\n\tpages = {dvdy.680},\n}\n\n
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\n Abstract Background Gastrulation is one of the most important events in our lives (Barresi and Gilbert, 2020, Developmental Biology , 12th ed.). The molecular mechanisms of gastrulation in multicellular organisms are not yet fully understood, since many molecular, physical, and chemical factors are involved in the event. Results Here, we found that one of muscle components, Troponin‐I (TnI), is expressed in future gut cells, which are not muscular cells at all, and regulates gastrulation in embryos of a sea urchin, Hemicentrotus pulcherrimus . When we block the function of TnI, the invagination was inhibited in spite that the gut‐cell specifier gene is normally expressed. In addition, blocking myosin activity also induced incomplete gastrulation. Conclusion These results strongly suggested that TnI regulates nonmuscular actin–myosin interactions during sea urchin gastrulation. So far, Troponin system is treated as specific only for muscle components, especially for striated muscle, but our data clearly show that TnI is involved in nonmuscular event. It is also reported that recent sensitive gene expression analysis revealed that Troponin genes are expressed in nonmuscular tissues in mammals (Ono et al., Sci Data , 2017;4:170105). These evidences propose the new evolutionary and functional scenario of the involvement of Troponin system in nonmuscular cell behaviors using actin‐myosin system in bilaterians including human being.\n
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\n \n\n \n \n Yaguchi, J., & Yaguchi, S.\n\n\n \n \n \n \n \n Rx and its downstream factor, Musashi1, is required for establishment of the apical organ in sea urchin larvae.\n \n \n \n \n\n\n \n\n\n\n Frontiers in Cell and Developmental Biology, 11: 1240767. August 2023.\n \n\n\n\n
\n\n\n\n \n \n \"RxPaper\n  \n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n  \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n\n\n\n
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@article{yaguchi_rx_2023,\n\ttitle = {Rx and its downstream factor, {Musashi1}, is required for establishment of the apical organ in sea urchin larvae},\n\tvolume = {11},\n\tissn = {2296-634X},\n\turl = {https://www.frontiersin.org/articles/10.3389/fcell.2023.1240767/full},\n\tdoi = {10.3389/fcell.2023.1240767},\n\tabstract = {Acetylcholine, a vital neurotransmitter, plays a multifarious role in the brain and peripheral nervous system of various organisms. Previous research has demonstrated the proximity of cholinergic neurons to serotonergic neurons in the apical organ of sea urchin embryos. While several transcription factors have been identified as playing a role in the development of serotonergic neurons in this region of a sea urchin,\n              Hemicentrotus pulcherrimus\n              , comparatively little is known about the specific transcription factors and their spatiotemporal expression patterns that regulate the development of cholinergic neurons. In this study, we establish the requirement of the transcription factor Rx for the development of cholinergic neurons in the apical organ of the species. Furthermore, we investigate the role of the RNA-binding protein Musashi1, known to be involved in neurogenesis, including cholinergic neurons in other organisms, and demonstrate that it is a downstream factor of Rx, and that choline acetyltransferase expression is suppressed in Musashi1 downregulated embryos. Our research also highlights the intricate network formed by neurons and other cells in and around the apical organ of sea urchin larvae through axons and dendrites, providing possibility for a systematic and complexed neural pattern like those of the brain in other organisms.},\n\turldate = {2024-04-04},\n\tjournal = {Frontiers in Cell and Developmental Biology},\n\tauthor = {Yaguchi, Junko and Yaguchi, Shunsuke},\n\tmonth = aug,\n\tyear = {2023},\n\tpages = {1240767},\n}\n\n
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\n Acetylcholine, a vital neurotransmitter, plays a multifarious role in the brain and peripheral nervous system of various organisms. Previous research has demonstrated the proximity of cholinergic neurons to serotonergic neurons in the apical organ of sea urchin embryos. While several transcription factors have been identified as playing a role in the development of serotonergic neurons in this region of a sea urchin, Hemicentrotus pulcherrimus , comparatively little is known about the specific transcription factors and their spatiotemporal expression patterns that regulate the development of cholinergic neurons. In this study, we establish the requirement of the transcription factor Rx for the development of cholinergic neurons in the apical organ of the species. Furthermore, we investigate the role of the RNA-binding protein Musashi1, known to be involved in neurogenesis, including cholinergic neurons in other organisms, and demonstrate that it is a downstream factor of Rx, and that choline acetyltransferase expression is suppressed in Musashi1 downregulated embryos. Our research also highlights the intricate network formed by neurons and other cells in and around the apical organ of sea urchin larvae through axons and dendrites, providing possibility for a systematic and complexed neural pattern like those of the brain in other organisms.\n
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\n \n\n \n \n Yaguchi, S., & Yaguchi, J.\n\n\n \n \n \n \n \n Development and function of nervous systems of sea urchin larvae.\n \n \n \n \n\n\n \n\n\n\n Hikaku seiri seikagaku(Comparative Physiology and Biochemistry), 40(3): 137–148. December 2023.\n \n\n\n\n
\n\n\n\n \n \n \"DevelopmentPaper\n  \n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n\n\n\n
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@article{yaguchi_development_2023,\n\ttitle = {Development and function of nervous systems of sea urchin larvae},\n\tvolume = {40},\n\tissn = {0916-3786, 1881-9346},\n\turl = {https://www.jstage.jst.go.jp/article/hikakuseiriseika/40/3/40_137/_article/-char/ja/},\n\tdoi = {10.3330/hikakuseiriseika.40.137},\n\tlanguage = {en},\n\tnumber = {3},\n\turldate = {2024-04-04},\n\tjournal = {Hikaku seiri seikagaku(Comparative Physiology and Biochemistry)},\n\tauthor = {Yaguchi, Shunsuke and Yaguchi, Junko},\n\tmonth = dec,\n\tyear = {2023},\n\tpages = {137--148},\n}\n\n
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